Uc merced scif stem cell instrumentation foundry 10,116 views. The tubes are in the rack at the side of the facs canto and run them in the order of bleach, facs rinse and deionised water. We have facsdiva, cellquest pro and flowjo analysis stations. Because many cytometer functions are controlled by bd facsdiva software, this guide also contains information about software features. Access after purchase you will be granted access to download a passwordprotected zipped file. Bd lsr ii users guide describes procedures necessary to operate and maintain your bd lsr ii flow cytometer. Bd facsaria ii quick reference manual pdf download.
Faculty core facility, li ka shing faculty of medicine. This users guide contains the instructions necessary to operate and maintain your bd facsaria flow cytometer. It is advisable to run a series of 3 cleans of 5 minutes each before starting. Rosenberg series in affective science series editors richard j. Facsaria sorp standard operation protocol basic operation.
About the system the bd facsverse system includes the bd facsverse cytometer, the optional bd facs universal loader, and bd facsuite software running on the system workstation. Standard operating procedure cytometry facility uzh pages 16 aria iii usz aug 2019 general remarks only power users having passed the sorter training at the cytometry facility are allowed to operate the aria iii cell sorter. Nur77 regulates nondeletional mechanisms of tolerance in t cells. With its unique feature set and technical easeofuse, the automated s3e cell sorter allows you to perform 14 color sorts right in your own lab. Multiple physical characteristics of each individual cellparticle are simultaneously measured, and expression levels of antigens can be detected using fluorescently labelled antibodies. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers.
Childrens healthcare of atlanta, emory university school of medicine, and georgia institute of technology have formed a close partnership that combines the clinical strengths of the nations largest pediatric healthcare provider childrens, the research strengths of a. Spacelabs new telemetry transmitter delivers a high performance product with a large full color screen. The miltenyi automacs pro is a powerful tool to make cell sorting easier. Lsr fortessa red switch on the wall behind the monitors fig. Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the users authority to operate the equipment. When contacting bd biosciences, have the following information available. If additional assistance is required, contact your local bd biosciences technical support representative or supplier. We are bostonlocated flow cytometry core facility equipped with analyzers 5laser bd lsr ii sorp, a facscanto and a facscalibur and cell sorters 4 laser16 color facs aria ii sorp and a 5 laser25 color moflo astrios.
Cell sorting on the facs aria ii is through service only the facs aria ii is only run by debby. Troubleshooting tips for successfully performing intracellular flow cytometry using cst recommended protocols and antibodies validated for flow. Fill the facs flow sheath solution and ethanol tanks to the tank seam, if needed. Lrb miltenyi automacs miltenyi automacs pro cell separator. We also provide consulting services on multicolor panel design and flow cytometry. Setup and tracking application guide for help troubleshooting. Bd facs accudrop beads are used to automatically set an accurate drop delay value. Starting up the cytometer follow these steps to start up your bd facsaria cytometer. Refer to the information supplied by the manufacturer for applicationspecific limitations. Bd facsaria ii users guide indiana university bloomington. Bd facs lsr fortessa user manual flow cytometry unit biomedical core facility technion faculty of medicine for help please call. The bd facsaria iii users guide assumes you have a working knowledge of basic microsoft. Cells that easily clump, agglutinate or settle out of suspension can be problematic when sorting, as this will cause clogging of the nozzle and disturbance of proper droplet formation. Product includes facs manual 527page pdf investigators guide 197page pdf scorechecker app example photos and videos.
Page 1 use this quick reference guide once you are familiar with the procedures in the bd facsaria ii users guide. Aria exus user manual rev b 1140201 7 relative humidity 0% 95% aria is designed to operate in a humid bathroom environment. Bd facsaria quick reference guide biosciences area. Fluorescenceactivated cell sorting facs has never been easier or more accessible. Bd facsaria quick reference guide use this quick reference guide once you are familiar with the procedures in the bd facsaria users guide. Each user must have completed and be current on the following ehs training. Manual of standard operating procedures uw pathology. Good morning, we have been trying to sort single hematopoietic stem cells very small population 1 cell 100k cells into both 72 well plates 10 ulwell and 96 well plates on the aria without much success and was wondering if anyone on the purdue list serv has had any luck with sorting small populations or single cell sorts on the aria.
Bd lsr ii users guide indiana university bloomington. Aria exus user manual rev b 1140201 1 getting started the fitbit aria wifi smart scale measures body weight and body fat percentage and automatically uploads this data to your online fitbit dashboard. Imaging flow cytometry and gst pulldown assays provide new insights into channel catfish leukocyte. Becton, dickinson and company bd is committed to providing a positive customer experienceour representatives knowledgeably provide technical support to address your product and service needs. A november 2007 bd biosciences benex limited canada japan nippon becton dickinson san jose, ca 9511807 bay k 1 ad toll free 888 2590187 company, ltd. Connected component masking accurately identifies the ratio of phagocytosed and cellbound particles in individual cells by imaging flow cytometry 2017 cytometry part a. Bd facschorus software optimizes the drop delay by sorting the accudrop beads and.
Spacelabs ariatele community, manuals and specifications. Browse our database of protocols for cell culture, nucleic acid, protein analysis, rna and dna applications, cloning, cell analysis, and drug discovery research. We have 1 bd facsaria manual available for free pdf download. See the troubleshooting section in the software or cytometer manuals. Accurately align the nozzle tip with the handsfree autogimbal system.
These limits are designed to provide reasonable protection against. Since 1979, the flow cytometry facility has provided university of iowa investigators with stateoftheart, laserbased instrumentation for basic cell research. Up to four leads of ecg, lead by lead, can be viewed along with the heart rate. Flow cytometry flow cytometry is a sophisticated technique that allows interrogation of few to millions of single cells or. Flow cytometry troubleshooting guide cell signaling. History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4. Achieve exceptional sort purity, recovery, and yield with the automated s3e cell sorter. A becton dickinson facs aria cell sorter with an aerosol management system was run in normal and failure modes using a radioac.
Ehs0735738 bloodborne pathogen training and annual refresher, ehs0745 hepatitis b medical surveillance training, ehs0604 hazardous waste generator training, and ehs0730 biohazardous waste training. Any type of cells, which can be easily kept in single cell suspension, can theoretically be sorted. For detailed information on software features, see the bd facsdiva. Researchers will prepare the samples and may assist with gating parameters. Using the automacs to remove cells from a sort sample that are not needed, fewer cells will need to be processsed in the actual sort which will take less time and provide better purity.
For more details, refer to chapter 4 in the users guide. Additionally, during a batch analysis, you can elect to process all tubes with different biexponential scales. View your patient s ecg or spo2 waveforms from the large easy to read graphic display. This guide shows a workflow that uses application settings. The instrument also conforms to the ul and cancsa general requirements 61010. The attune nxt flow cytometer is a class i laser product per cdrh regulations and eniec 60825. Page 1 bd facscalibur instructions for use for in vitro diagnostic use part no. What the face reveals basic and applied studies of spontaneous expression using the facial action coding system facs second edition. Bd facs canto ii flow cytometer quick user guide v. The facility provides a wide variety of cell analysis and sorting services. Top up the tubes from the wash bottles if required.
Becton dickinson facs aria fusion cytek aurora with 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm lasers becton dickinson lsr ii with 405nm, 488nm, 561nm, and 639nm lasers. Facial action coding system facs, second edition edited by paul ekman and erika l. Faqs for cell sorting what types of cells can i sort. Unused sample and sorted cells will be returned to the researcher upon completion of the sort. For detailed information on software features, refer to the bd facsdiva. Powerful automation that enables pushbutton startup and reproducible setup regardless of user experience. Aria exus user manual rev b 1140201 european union eu.
Bd facsaria users guide national center for xray tomography. Pediatric research is thriving and expanding in atlanta through a unique, multiinstitutional initiative. This equipment has been tested and found to comply with the limits for a class a digital device, pursuant to part 15 of the fcc rules. Amir grau 0542100778 yaakov sakoury 0507498565 ofer shenker 0524568846 a. Can we use 1x pbs or diionized water as sheath fluid for.